Analysis Note

ControlPOSITIVE CONTROL CELLS: PC12, Ntera2.

Application

Research CategoryNeuroscience

Anti-Internexin Antibody, α is an antibody against Internexin for use in ELISA, WB, IC, IH.

Immunohistochemistry on formalin or paraformaldehyde fixed tissues.

Immunocytochemistry.

Western blot: using human or rat brain homogenates.

ELISA

Optimal working dilutions must be determined by end user.

Research Sub CategoryNeurofilament & Neuron MetabolismNeuronal & Glial Markers

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Full length recombinant rat alpha-internexin fused to E. coli Trp E.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1032

Physical form

Serum liquid, containing no preservatives.

Specificity

alpha-internexin, C-terminal globular "tail" region. By western blot, AB5354 detects a major band at 66 kDa and a minor band at approximately 150 kDa corresponding to alpha internexin monomer and dimer, respectively. A minor band at approximately 50 kDa is occasionally detected and is believed to be a degradation product of alpha internexin. This antibody has been referred to in the literature as the R36 antibody (Evans, 2002).

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.